Grades

•65% of 650 = 423
•90% of 350 =315
•20 extra credit
•
•Total=758 or 75.8%   =C
 

 

 
Today’s plan
 
DNA replication animation
Continue with chapter 8 - Genetics
 
UNIT 3 - Microbial Genetics and Viruses

 

I. Microbial Genetics
A. Background
B. Structure of DNA
C. DNA replication
D. DNA to protein
1. Transcription
2. Translation
 

 

 

Fig. 8.7

Prokaryotic DNA replication
Typically one circular chromosome
• One origin of replication (euks have many)
• Takes longer than 20 minutes, how is that possible?
 
In log phase cells DNA replication begins again before
the separation of the 2 daughter cells
 

Fig. 8.6

Origin of replication
• Replication fork
• Leading strand - continuous
• Lagging strand - discontinuous
RNA polymerase
• RNA primer
• DNA polymerase
• DNA ligase
 

Animations

 

DNA replication

http://biotech-adventure.okstate.edu/low/basics/dna_replication/replication4/animation/

 
 
Transcription
 

http://www-class.unl.edu/biochem/gp2/m_biology/animation/m_animations/gene2.swf

 
 
Translation
 

http://www-class.unl.edu/biochem/gp2/m_biology/animation/m_animations/gene3.swf

 

 

Fig. 8.8

 
Transcription
Still 5’ --> 3’
-Promoter to
start the process
-Coding Region
mRNA is made
Copy of DNA sequence
-Terminator
 

Fig. 8.9

Codons
Degeneracy
Nonsense codons
Dual function-Methionine/start codon

 

Fig. 8.10

Translation

 

 

Fig. 8.11
Transcription and translation occur simultaneously in prokaryotes.
 
Why not in eukaryotes?

Transcription take place in the nucleus in euks