BIO 577 HEMATOLOGY and Immunohematology SYLLABUS
Dr. Fred McCorkle OFFICE HOURS:
Winter 2004 Brooks 114 M 1-3 pm
774-3348 T 1-3 pm
or by appt.
This course is designed to meet the requirement for our medical technologist students to enter their clinical training program. The students will study current concepts of hematopoiesis, including red blood cell and white blood cell morphogenesis, physiology, pathophysiology, blood banking and immunohematology. The laboratory will introduce the student to clinical hematologic evaluations, including clinical techniques and procedures and blood typing.
A. OBJECTIVES
The objective of a hematology course is to introduce the student to the current concepts of hematopoiesis, including red blood cell and white blood cell morphogenesis, physiology and pathophysiology and blood banking and blood typing. The laboratory will cover the basic techniques and procedures commonly used in clinical hematologic evaluation.
B. TEXTBOOK
Hematology: Clinical Principles and Applications by Rodak, 2 edition, W.B.Saunders Company
Basic & Applied Concepts of Immunohematology by Kathy D. Blaney and Paula R. Howard , 1 ed., Mosby
C. REFERENCES
Fundementals of Hematology - Rifkind, Bank, Marks, Nossel, Ellison, and Lindenbaum
Clinical Hematology and Fundamentals of Hemostasis - Pittiglio and Sacher
Laboratory Medicine Hematology - Miale
Hematology - Beck
Essential Hematology - Hoffbrand and Pettit
Lecture Notes on Hematology - Hughes and Jones
D. EVALUATION
2 Lecture Examinations (10% Each) 20%
2 Laboratory Examinations (10% Each) 20%
Weekly Laboratory Reports 10%
Library Report DUE APRIL 8, 2004 10%
Final Lecture Examination (Comprehensive) 40%
The library report will be a synopsis dealing with a specific area of hematology based upon information from at least 5 original research articles from current scientific journals. A photo-copy of the first page of each article will be included with the paper. This paper should be no less than 5 typed pages. Papers turned in late (up to three days) will automatically receive a 10% reduction in grade per day. Those over three days will NOT be accepted. This paper can not have been used in any other course.
Graduate students must turn in a 1-2 page review of a current research article in hematology by April 8, 2004 to get graduate credit for this course, include a copy of the article.
Date Tentative Lecture
Jan. 15 1. Hematopoiesis
20 2. Reticuloendothelial System
22 3. Normocytic Anemias
27 4. Megaloblastic Anemias I. Vitamin B12 Deficiency
29 5. Megaloblastic Anemias II. Folic Acid Deficiency
3 6. Hypochromic Anemias I. Iron Deficiency
5 7. Hypochromic Anemias II. Heme Metabolism
Feb 10 8. Hemoglobin I.
12 9. Hemoglobin II.
17 10. *****EXAMINATION*****
Feb. 19 11. Hemolytic Anemias I. Introduction
24 12. Hemolytic Anemias II. Immunohemolytic Anemias
26 13. Hemolytic Anemias III. Membrane Disorder
2 14. Hemolytic Anemias IV. Metabolic Disorders
4 15. Blood Groups I. Physiology
Mar. 6-14 ********SPRING BREAK********
Mar. 16 16. Blood Groups II. Transfusion Therapy
18 17. Blood Typing and Blood Banking
23 18. Leukocytes I. Physiology
25 19. Leukocytes II. Phagocytosis
30 20. Leukocytes III. Pathology
1 21. *****EXAMINATION*****
Apr. 6 22. Leukemias & Polycythemias
8 23. Malignant Lymphomas I. Clinical Aspects
13 24. Malignant Lymphomas II. Pathology
15 25. Plasma Cell Disorders
20 26. Histocytoses and Lipidoses
22 27. Hemorrhagic Disorders I. Protein Interactions in the Clotting Mechanisms
27 28. Hemorrhagic Disorders II. Platelets
29 29. Hemorrhagic Disorders III. Disorders of Primary and Secondary Hemostasis
May 6 30. *****FINAL EXAMINATION*****
Thursday 10:00-11:50 am
TENTATIVE HEMATOLOGY LABORATORIES
DATE LABORATORY
Jan 15 1. White cell count. Student does his/her own blood count, and repeats several times and they do a known and unknown blood sample to determine how close they can come to machine values. The student is reintroduced to microscopes and to counting chambers. Using the Unopette system. Pages 89-95.
Jan 22 2. Red cell count. Student does her/his own blood, Using the Unopette system. pages 95-97.
Jan 29 3. . Laboratory practical examination -- Students are given an unknown blood sample to determine white cell and red cell counts using the Unopette system. this is usually repeated several times for accuracy.
5 4. Platelet Count and Reitulocyte Count. Tests will be performed on their own blood. pages 111-
118.
Feb 12 5. Hemoglobin determination, and Mean corpuscular Values Rate. Tests are performed on their own blood
pages 124-126.
Feb 19 6. Sickle cell examination and Anemias. Students learn two types of test for sickling and examine slides of various types of anemias. AND/OR
Eosinophil count. Pages 119-122.
Feb 26 7. Sedimentation rate and Hematocrit. Tests are performed on their own blood and unknown, and should also repeat hemoglobin determination and mean corpuscular values. pages 107-111.
4 8. Stained red cell examination. Student learns red cell morphology of his/her own blood .
pages 97-102.
Mar 6 - 14 ********SPRING BREAK********
Mar 18 9. Blood Typing and Testing.
Mar 25 10. Laboratory Practical Examination -- Students are given an unknown blood sample to examine for red cells morphology, hemoglobin values, mean corpuscular values, sickle cell, sedimentation rate, hematocrit, platelet and reitulocyte.
1 11. Differential white cell count. Students learn to identify the various white cells on smears, does their own differential count.
Apr 8 12. Studies of Unknown Blood Smears
Apr 15 13. Studies of Unknown Blood Smears
Apr 22 14. Studies of unknown Blood Smears
Apr 29 15. Laboratory Practical Examination on Blood Smears.
CMU provides students with disabilities reasonable accommodation to participate in educational programs, activities or services. Students with disabilities requiring accommodation to participate in class activities or meet course requirements should first register with the office of Student Disability Services (250 Foust Hall, telephone #517-774-3018, TDD #2568), and then contact me as soon as possible.
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Policy on Academic Integrity: In may 2001, the Central Michigan University Academic Senate approved the Policy on Academic Integrity which applies to all university students. Copies are available on the CMU website at and in the Academic Senate Office in room 108 of Bovee University Center. All academic work is expected to be in compliance with this policy.
Library Paper for Hematology
The purpose of this assignment is to introduce you to the primary literature of hematology and to encourage critical thinking skills. The paper is due April 19, 2001.The library paper will be on a topic of your own choosing but must be on a Hematology topic.. T Write a 5-6 page library paper using at least 5 original research articles (12-pt. double-spaced, 1" margins) including a short introduction. Review articles may be used as these synthesis the experiments of others rather than describing original experiments but these review articles will not count as original research articles. Thus, there are no methods and materials or results sections in review articles. Research articles describe original experiments that the authors have done, and usually follow the standard format of Introduction, Materials and Methods, Results, Discussion, References. The exceptions are research articles published in Science or Nature magazines; in these journals, to save space the methods are briefly described in either the figure legends or in footnotes, and the article is not broken up by section subheadings. The main body will address the research of the these papers on your topic.
Title format: Title.
Introduction: Do not use heading, do restrict to one or two paragraphs. The textbook should not be referenced. Remember to reference all this work as it is not your work. This is true for this whole paper.
Body: Do not use heading, but start a new paragraph with an introductory sentence such as, "In the study by Frye and Edidin (1970), ..." Explain any unusual techniques used; if you don't understand the methods, do some research to find out about them. Address the hypothesis of the experimenters, the specific experiments to test the hypothesis, and the conclusions drawn. What controls were done? Were you convinced by the results? How strong was their experimental evidence? Did the authors over-interpret their data in the Discussion section?
References: Use a heading for this section only. Alphabetize by first author. Provide complete author list, date, title, journal, volume, and pages.
Citation style: Use (Hertzler, 1998) for one author, (Hertzler and McCorkle, 1998) for two authors, and (Hertzler et al., 1998) for multiple authors within the text. Use (reviewed by Hertzler, 1998) to reference your review in the first paragraph. Use a citation after the first sentence of your research paper discussion, and again whenever a major finding or conclusion is made. Do not cite page numbers. Papers with missing citations will receive a Zero.
Spelling and Grammar: Use but don't rely on the spell-checker on your computer. Always proofread. Points will be lost for sloppy proof reading.
Plagiarism: Do not use quotation marks to cite passages. Put things in your own words. You may think the authors expressed their sentence in the best possible way, but you must rephrase it, and correctly attribute it. I will check the reference if in doubt, and plagiarized papers will receive a Zero with the possibility of further action through the Office of Student Life. If you are unsure of what constitutes plagiarism, consult the handbook PLAGIARISM: A BRIEF OVERVIEW.
Library Use of articles: You must either use references in the library or make a photocopy. Ripping out research articles from journals in the library is a scholarly crime as well as theft and destruction of state property, and will be addressed accordingly.
Library Paper Grading: This paper will be worth 100 points. Papers received late will receive a 10 % per day reduction, starting after the class period on the day due and will not be accepted after three days. Do not use personal pronouns in your paper ( they, I , He or She). You will lost one point each time you use these.