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SYLLABUS
BIO 576 ANIMAL CELL CULTURE Fall, 1999
Dr. Fred McCorkle Office Hours: Brooks 114 Mon: 10-12 am 774-3348 Wed: 10-12 am 1-2 pm Or by appt.
OBJECTIVES The objective of this course is to teach the student the practical aspects of animal cell culture techniques which include, media preparation, aseptic techniques, routine cell maintenance and the freezing of cells for storage.
METHODOLOGY The emphasis of this course will be on the practical aspects of cell culture techniques. These techniques will involve media preparation, aseptic manipulation of cell culture, routine cell maintenance and cell storage. This will be a laboratory course with 1 hour of lecture and 5 hours on lab day plus 3-4 hours per week to be arranged. Most labs will take over several weeks to complete.
TEXTBOOK AND LABORATORY MANUAL
Freshney, R. Ian. 1988. A Manual of Basic Technique, Alan R. Liss, Inc., New York. Additional lab experiments will be provided.
EVALUATION Laboratory Notebook - 20% Total Grade Term Paper - 20% Total Grade Term Paper Due - Nov. 9, 1999. The term paper will be a synopsia dealing with a specific area of cell culture based upon information from at least 5 original research articles from current scientific journals. A photocopy of the first page of each article will be included with the paper. This paper should be no less than 5 typed pages. Papers turned in late ( up to three days) will automatically receive a 10% reduction in grade. Those over three days will NOT be accepted. Laboratory - 60% Total Grade Demonstration of techniques and lab reports - 40% Cooperation and preparation - 20% Successful completion of special project - 40% Projects will be done individually. Each student will develop, complete and write up a special project. Example: Effect of a drug on a cell culture.
LAB SCHEDULE - Tentative
Lab 1 - Introduction to biology of the Cultured Cell, equipment, aseptic, technique, media preparation and sterilization. Lab 2 - Disaggregation of tissue and Primary Culture Lab 3 - Maintenance of the culture - Cell Lines Lab 4 - Cell Viability Lab 5 - Preservation of Animal Cells by Freezing Lab 6 - Primary culture of embryonic chick fibroblasts Lab 7 - Primary culture from explants Lab 8 - Organ Culture Lab 9 - Primary mouse embryo cultures Lab 10 - Primary culture of rat myocardial cells Lab 11 - Culture of specific cell types Lab 12 - Lymphocyte preparation and blast transformation
BIBLIOGRAPHY
Freshney, R.I. Animal Cell Culture: A Practical Approach. 1986. IRL Press, Oxford.
Jakoby, W.B. and Pastan, I.H. Cell Culture: Methods in Enzymology, Vol 58. 1988. Academic Press, San Diego.
Baserga, R. Ed. Cell Growth and Division: A Practical Approach. 1989. IRL Press, Oxford.
Cormier, F. and Dieterlen, F. Long-term cultures of chicken bone marrow cells. Lievre, 1990. Exp. Cell Research 190: 113-117.
RELEVANT PERIODICALS
Cell Tissue Research Cell Science Nature Journal of Immunology Thymus International Journal of Cell Cloning Blood Hybridoma
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