Chapter Objectives

Chapter 11

DNA Technology and the Human Genome

1.  Describe the Human Genome Project and the current implications to man using DNA technology.

2.  Describe and distinguish between transformation, transduction, and conjugation.

3.  Describe the basic process by which plasmids are used to customize bateria.

4.  Explain how restriction enzymes and DNA ligase are used to create recombinant DNA.

5.  Explain how DNA synthesizing and DNA sequencing machine work.

6.  Explain how gel electrophoresis is used to sort DNA and protein molecules.

7.  Explain how the polymerase chain reaction (PCR) can be used to amplify (clone) a segment of DNA.

8.  Explain how genetic engineering has helped produce human insulin, human growth hormone, and various vaccines.

9.  Describe the progress and potential for gene therapy in humans.

10.  Describe the potential benefits, risks, and concerns of producing genetically modified microbes, crops, farm animals, and humans.

11.  Explain how DNA sequencing has affected our understanding of the evolutionary connections of life.